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Most gene mutations and biologically active molecules cause complex responses in animals that cannot be predicted by cell culture models. Yet animal studies remain too slow and their analyses are often limited to only a few readouts. Here we demonstrate high-throughput optical projection tomography with micrometer resolution and hyperdimensional screening of entire vertebrates in tens of seconds using a simple fluidic system. Hundreds of independent morphological features and complex phenotypes are automatically captured in three dimensions with unprecedented speed and detail in semi-transparent zebrafish larvae. By clustering quantitative phenotypic signatures, we can detect and classify even subtle alterations in many biological processes simultaneously.

We term our approach hyperdimensional in vivo phenotyping (HIP). To illustrate the power of HIP, we have analyzed the effects of several classes of teratogens on cartilage formation using 200 independent morphological measurements and identified similarities and differences that correlate well with their known mechanisms of actions in mammals. Download Video Seni Tunggal Baku Ipsi. Introduction Large-scale screening of phenotypes induced by small molecules, natural products, gene mutations, and other agents is essential for all fields of modern biology and drug discovery. Although cell-based assays are amenable to high-throughput screening (HTS), results often fail to translate to animal models and clinical trials.

Download Lagu Gudang Lagu Tarling Cirebonan Terbaru 2017. Even the most sophisticated in vitro models fail to preserve the complexity and architecture of intact organs and in vivo processes such as disease pathology, tissue homeostasis, and drug-induced toxicity. To address these limitations, chemical screens are increasingly being conducted using zebrafish ( Danio rerio), a small vertebrate model organism that is widely used for genetic screens and amenable to high-throughput methodologies.